Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters

摘要

Simplified schematic representation of the E. coli strain DH5α transfection. a Recombinant plasmid (pGL3-luc/pbr biosensor).b Recombinant plasmid (pGL3-luc/cad biosensor). pGL3-luc/pbr biosensor and pGL3-luc/Cad biosensor were transferred to the E. coli strain DH5α using the chemical method of CaCl2 and then were screened using selective plates containing antibiotic ampicillin. c Sequencing and integrity of synthesis sequence. dPGL3-luc/pbr biosensor pGL3-luc/cad biosensor. The promoter region was sequenced in the received plasmid