c-Myc promotes tubular cell apoptosis in ischemia-reperfusion-induced renal injury by negatively regulating c-FLIP and enhancing FasL/Fas-mediated apoptosis pathway

摘要

c-Myc is upregulated in the kidneys of I/R rats and H/R-treated NRK-52E cells. SD rats were subjected to 60 min ischemia and 24 or 48 h reperfusion. a RT-PCR was performed to assay the levels of c-Myc mRNA in the kidneys (six rats per group). b Western blotting was performed to visualize c-Myc expression in kidneys (six rats per group). c The levels of c-Myc were visualized using immunohistologic analysis in the kidneys of control and 24 h reperfusion respectively (six rats per group). Bar = 100 μm. Original magnification ×200 or ×400. d The protein levels of c-Myc were assayed by Western blotting in NRK-52E cells with or without 6 h hypoxia and 3 or 6 h reoxygenation treatment (three experiments per group). Data are presented as the mean ± SEM. **P < 0.01 versus control