High-resolution spectral information enables phenotyping of leaf epicuticular wax in wheat

摘要

Variation in a absorbance, b transmittance and c reflectance derived by the removal of the EW coat with HPLC chloroform (CHCl3). These variations are presented as the difference of the spectral signature of the leaf after the wax coat was removed minus the the spectral response of the leaf with the wax coat in place. The red solid lines define the red edge and the dash line mark the end of the visible and start of the NIR region. The slope of the linear regression models (d) were fitted as Y = a + bX, where Y corresponds to the independent variable EW (mg·dm−2), X is the percentage of light reflectance at one nanometer resolution, a and b are the intercept and the slope of the fitted model, respectively. The statistical significance of the models was P≤0.05 or less