Effects on Some Therapeutical Biochemical and Immunological Parameters of Honey Bee (

摘要

Various negative factors contribute to a decline in insect pollinators. The aim of this study was to assess the impact of commercial probiotic EM® PROBIOTIC FOR BEES on honey bees. The study was conducted in field and laboratory-controlled conditions. In the field, the sugar syrup with 10% of probiotic was administered by spraying or feeding the honey bee colonies in order to evaluate the colonies’ strength and Nosema spp. infection levels. In the laboratory, the adult workers have been fed with sugar syrup supplemented with 2.5, 5, and 10% of EM® for bees for biochemical and immunological analyses of hemolymph, and with 5 and 10% for measuring the size of hypopharyngeal glands. It was found that following the EM® for bees administration the Nosema spp. spore counts in colonies were significantly reduced, and colonies’ strength was increased. The results at the individual level showed positive physiological changes in treated groups of adult bees, but, at the same time, a higher mortality rate. Our findings indicate that the EM® for bees is a promising food additive for nosemosis combating. Therefore, additional emphasis needs to be placed on studies investigating the nutritional requirements crucial to improve and sustain honey bee colonies health.