IKKε isoform switching governs the immune response against EV71 infection

摘要

a IKKε is located at {"type":"entrez-nucleotide","attrs":{"text":"NC_000001.11","term_id":"568815597","term_text":"NC_000001.11"}}NC_000001.11 (206470243..206496890) of chromosome 1 in GRCh38. Each exon coverage of IKKε is illustrated and the isoform switching occurs in exon 20 at 4 and 8 h.p.i. compared with mock infection group. b IKKε isoform switching is validated by droplet digital PCR (ddPCR). IKKε v2 was upregulated while IKKε v1 was downregulated in response to EV71 infection determined by NGS (left panel for RD cells) and ddPCR (middle panel for RD cells and right panel for SH-SY5Y cells). The proportion of each IKKε isoform was indicated. c IKKε isoform switching is confirmed by western blotting. The Click-iT AHA assay was performed to measure newly synthesized IKKε v1 and v2. The synthesis of IKKε v2 was increased while IKKε v1 was decreased in EV71 infection. The upper panel of IP-Streptavidin is a long exposure while the lower panel is a short exposure.