Retro-orbital injection of FITC-dextran combined with isolectin B4 in assessing the retinal neovascularization defect

摘要

The effects of different concentrations of FITC-dextran in P17 OIR mice following retro-orbital (RO) injection. a: The fluorescence of small vessels and neovascular tufts following administration of 0.05 ml of 12.5 mg/ml FITC-dextran was too weak, whereas these structures were fully observed in the presence of 0.05 ml of 25 mg/ml and/or 50 mg/ml FITC-dextran. Original magnification, 50×; b: Quantification of retinal neovascularization (NV) in P17 OIR mice among the three groups. Compared to 12.5 mg/ml FITC-dextran, 25 mg/ml and 50 mg/ml FITC-dextran showed a significant increase in the NV/total retinal area% (P < 0.05). (*P < 0.05, b) n = 9 from 9 mice