Relationships between Seminal Plasma Metabolites Semen Characteristics and Sperm Kinetics in Donkey (

摘要

A deeper knowledge of reproductive biology may be helpful in the donkey to avoid the risk of extinction that some breeds are facing. The evaluation of metabolites in seminal plasma provides crucial information for the knowledge of donkey sperm metabolism, for obtaining comparative information with other species, as well as for providing useful elements for the formulation of extenders for sperm dilution and conservation. Moreover, correlations of seminal metabolites with sperm kinetics highlight new possible markers of sperm quality. Using multivariate analysis, all metabolic, seminal, and spermatic data were merged in a single dot that grouped individual stallions within clusters in the Cartesian axes according to the different spermatic characteristics. This amount of information also allows to shed light on the effects of total or partial removal of seminal plasma for improving sperm preservation. The inclusion in the study of an azoospermic individual represents a further discriminating element in the analysis of sperm quality under physiological and pathological conditions.