Periplasmic synthesis and purification of the human prolactin antagonist Δ

摘要

SDS-PAGE under non-reducing conditions and Western Blotting with samples of periplasmic fluid obtained from E. coli BL21(DE3) by osmotic shock, after 5 h of cultivation with 0.4 mM IPTG at different temperatures: 25 °C, 30 °C, 32 °C, 35 °C and 37 °C (a) and at 25 °C with different IPTG concentrations: 0.2 mM, 0.4 mM, 0.6 mM, 0.8 mM and 1.0 mM. b Lane 1: molecular mass marker, lane 2: recombinant human prolactin internal standard (23 kDa). The arrows indicate the position of Δ1-11-G129R-hPRL (22 kDa)