The Influence of Single Tandem and Clustered DNA Damage on the Electronic Properties of the Double Helix: A Theoretical Study

摘要

Oxidatively generated damage to DNA frequently appears in the human genome as the effect of aerobic metabolism or as the result of exposure to exogenous oxidizing agents, such as ionization radiation. In this paper, the electronic properties of single, tandem, and clustered DNA damage in comparison with native -DNA are discussed as a comparative analysis for the first time. A single lesion—8-oxo-7,8-dihydro-2′-deoxyguanosine (G ), a tandem lesion—(5′ ) and (5′ ) 5′,8-cyclo-2′-deoxyadenosine (cdA), and the presence of both of them in one helix turn as clustered DNA damage were chosen and taken into consideration. The lowest vertical and adiabatic potential (VIP ~ 5.9 and AIP ~ 5.5 eV, respectively) were found for G , independently of the discussed DNA lesion type and their distribution within the double helix. Moreover, the VIP and AIP were assigned for -trimers, - dimers and single base pairs isolated from parental -hexamers in their neutral and cationic forms. The above results were confirmed by the charge and spin density population, which revealed that G can be considered as a cation radical point of destination independently of the DNA damage type (single, tandem, or clustered). Additionally, the different influences of cdA on the charge transfer rate were found and discussed in the context of tandem and clustered lesions. Because oligonucleotide lesions are effectively produced as a result of ionization factors, the presented data in this article might be valuable in developing a new scheme of anticancer radiotherapy efficiency.