A sequential methodology for the rapid identification and characterization of breast cancer-associated functional SNPs

摘要

Reel-seq library construct. Reel-seq to identify fSNPs. SNP1: a non-fSNP exhibiting a balanced gel shift pattern between risk and non-risk allele (left in purple); therefore, showing no difference in the ratio of risk and non-risk allele (right in purple) between the buffer-treated control and the NE-treated sample. SNP2: fSNP demonstrating allele-imbalanced gel shift pattern between the risk and non-risk allele (left in blue); therefore, showing significant difference between the buffer-treated control and the NE-treated sample (right in blue). Nonspecific shifting is not shown on EMSA, but it is considered to be evenly and randomly distributed. EMSA electrophoresis mobility shift assay, NE nuclear extract, NGS next-generation sequencing.