Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts

摘要

Adipocyte Conditioned Medium Promotes Fibroblast Scratch Closure. ( ) Representative 10x DIC images at 0 and 24 hours of human dermal fibroblasts in control medium, pre-adipocyte conditioned medium (PCM) and adipocyte conditioned medium (ACM), respectively. Visually identified scratch boundaries are demarcated in red. Scale bar is 500 µm. ( ) Quantification of percent scratch closure. Data is presented as mean SEM (n = 6 independent experiments with four samples per group). A two-way ANOVA with Dunnett multiple comparison tests at each time point indicate that fibroblasts exposed to ACM closed the gap significantly faster than fibroblasts in control medium (**p<0.01, *p<0.05). ( Cell number after 48 hours was not significantly different between groups, indicating that ACM does not enhance proliferation but promotes fibroblasts closure of the scratch.