The cellular heat shock response monitored by chemical exchange saturation transfer MRI

摘要

( ) Contribution of CEST signals from medium, collagenized MCAs and cells to the total Z-spectrum and AREX spectrum (B  = 9.4 T and B  = 0.8 µT). The CEST signals mainly originated from the 3D cell culture of 16–18·10 HepG2 cells, while proteins in medium and collagenized MCAs contributed only to a small background signal (Table ). ( ) rNOE AREX images of the different contributions to the −3.5 ppm rNOE signal overlaid on a H RARE image. In the two images without cells, a small rNOE signal across the bioreactor was observed, while the presence of cells led to distinct rNOE signals in the expected size and location of the cavity structure on the MCAs.