Dual-targeting nanoparticle vaccine elicits a therapeutic antibody response against chronic hepatitis B

摘要

, Schematic illustration of ferritin-based vaccine delivery vehicle. , Size exclusion chromatography of SpyTag–ferritin NPs. The ultraviolet absorptions at 280 nm and 260 nm are shown. AU, absorbance unit. , SDS–polyacrylamide gel electrophoresis analysis of the covalent ligation of SpyTag–ferritin (~22.4 kDa) and SC–preS1 (~25.4 kDa) at 1:0.5, 1:1 and 1:2 mole ratios at 4 °C overnight. , Size exclusion chromatography of ferritin NP–preS1. , TEM images and two-dimensional reconstruction of SpyTag–ferritin NP and ferritin NP–preS1. Panels – show representative results of at least three independent experiments. , Anti-preS1 response at vaccine immunization on day 21 was analysed using enzyme-linked immunosorbent assay (ELISA) (  = 3 mice per group). Data are representative of five independent experiments. , Kinetics of anti-preS1 levels after immunization (  = 3). The red arrows indicate the immunization times. , Comparison of conjugated and unconjugated vaccines on anti-preS1 response. , Anti-preS1 IgG avidity on vaccine immunization was detected with ELISA. Each curve represents an individual serum sample (  = 3) (left). The slopes of each fitting curve were calculated (right). OD, optical density. , Titres of preS1-specific IgG1 and IgG2c in the sera from ferritin NP–preS1 vaccine (  = 8) or SC–preS1 (  = 14) immunized mice on day 21. – show representative results of three independent experiments. In – , data are shown as mean ± s.e.m.; statistical significance was determined using an unpaired two-tailed -test. .