Elucidation of proliferative capability of mononuclear tetraploid cells emerging spontaneously from diploid cells using image cytometry and fluorescence in situ hybridization

摘要

Proliferation of tetraploid cells ( s) emerging from diploid cells is considered to be a critical event toward tumourigenesis, or cancer progression. Recently, several studies have reported that binuclear s emerging from normal cells are capable of mitosis, however, it has not been confirmed directly whether mononuclear s emerging from normal cells could proliferate, even cancer cells. The aim of this study is to detect mononuclear s , spontaneously emerging from diploid cells and to elucidate their proliferative capability directly. For this purpose, we have developed a novel method.