Immunoassays for pentamidine and related compounds: Development of a facile inhibitory ELISA suitable for clinical use

摘要

Aromatic dicationic drugs have a broad spectrum of activity against protozoal and fungal pathogens including and Pentamidine serves as the exemplar for an extensive collection of newly synthesized related compounds, which have reduced toxicity and a wider range of target organisms. Assays of pentamidine and related compounds have depended on HPLC‐tandem mass spectrometry (HPLC‐TMS) for the quantitation and identification of drug and metabolites. Immunoassays for pentamidine would have many advantages over the HPLC methods including relative simplicity of assay format and required equipment, convenience in sample preparation and reduction in time and cost of assays.