Salmonella-Infected Aortic Aneurysm: Investigating Pathogenesis Using Salmonella Serotypes

摘要

infection is most common in patients with infected aortic aneurysm, especially in Asia. When the aortic wall is heavily atherosclerotic, the intima is vulnerable to invasion by , leading to the development of infected aortic aneurysm. By using THP-1 macrophage-derived foam cells to mimic atherosclerosis, we investigated the role of three serotypes – Typhimurium, Enteritidis, and Choleraesuis – in foam cell autophagy and inflammasome formation. Herein, we provide possible pathogenesis of -associated infected aortic aneurysms. Three serotypes with or without virulence plasmid were studied. Through Western blotting, we investigated cell autophagy induction and inflammasome formation in -infected THP-1 macrophage-derived foam cells, detected CD36 expression after infection through flow cytometry, and measured interleukin (IL)-1β, IL-12, and interferon (IFN)-α levels through enzyme-linked immunosorbent assay. At 0.5 h after infection, plasmid-bearing . Enteritidis OU7130 induced the highest foam cell autophagy – significantly higher than that induced by plasmid-less OU7067. However, plasmid-bearing Choleraesuis induced less foam cell autophagy than did its plasmid-less strain. In foam cells, plasmid-less infection (particularly . Choleraesuis OU7266 infection) led to higher CD36 expression than did plasmid-bearing strains infection. OU7130 and OU7266 infection induced the highest IL-1β secretion. OU7067-infected foam cells secreted the highest IL-12p35 level. Plasmid-bearing Typhimurium OU5045 induced a higher IFN-α level than did other serotypes. serotypes are correlated with foam cell autophagy and IL-1β secretion. may affect the course of foam cells formation, or even aortic aneurysm, through autophagy.