The Effects of EGTA on the Quality of Fresh and Cryopreserved-Thawed Human Spermatozoa

摘要

Sperm cryopreservation-thawing process has damaging effects on the structure and function of sperm, namely cryoinjury.Calcium overload has been reported as a postulated mechanism for sperm damage during the first steps after thawing.This study was designed to assess the intracellular calcium (Ca ) after cryopreservation and to clarify the roleof a calcium chelator ethylene glycol-bis (2-aminoethyl ether)-N, N, N′, N′-tetraacetic acid (EGTA) on human sperm quality.