The Addition of Lactobacillus spp. Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen

摘要

is an important infectious agent in cattle. The pathogen may cause a wide range of clinical signs, including mastitis, arthritis, pneumonia and reproductive disorders. Artificial insemination with contaminated semen may be a source of infection in infection-free areas or herds. Hence, the antimicrobials used in the preparation of seminal doses should be re-evaluated, or alternative measures to antimicrobials should be tested. This in vitro study aims to evaluate novel strategies to reduce the risk of the transmission of the pathogen through contaminated semen during artificial insemination. Hence, we assess the effect of the addition of (1) the antimicrobial enrofloxacin, (2) the antimicrobial doxycycline, or (3) a probiotic of human origin that contains acid lactic bacteria of the genus , on the viability of in diluted bull semen in a Tris-citrate-fructose medium. The data show that the pathogen is negatively affected by the addition of 0.125 μg/mL of enrofloxacin, 0.0625 μg/mL of doxycycline, or the probiotic at a concentration of 3.24 × 10 or 3.24 × 10 colony-forming units/mL in diluted semen. Our results are promising in the field, as they may support new strategies to reduce the risk of the transmission of through artificial insemination.