Phylogenetic Position of Pleurogenoides Species (Plagiorchiida: Pleurogenidae) from the Duodenum of Indian Skipper Frog Euphlyctis Cyanophlyctis (Amphibia: Dicroglossidae) Inhabiting the Western Ghats India

摘要

Two species of digenetic trematodes of the genus ., and have been described from India. Information regarding the molecular data of various species of the genus Travassos, 1921 is virtually lacking. This study addresses the application of molecular markers to validate the phylogenetic position of and . In the present study, two species and were collected between January 2016 to October 2017, infecting the freshwater frogs inhabiting the Western Ghats, India. In the present study, the two species were identifi ed morphologically and by PCR amplification of the 28S ribosomal RNA gene. Phylogenetic tree results clearly demonstrate that both and belongs to the family Pleurogenidae . Based on these results, we presented and discussed the phylogenetic relationships of and within family Pleurogenidae from India. Phylogenetic analyses showed that and cluster according to their vertebrate host and revealed an important congruence between the phylogenetic trees of and of their vertebrate hosts. and clearly constitute a separate, sister branch with other species of the genera, ( ) and . The present study firstly provides important information about the molecular study and phylogenetic analysis of and . This study will also serve as a baseline for species identifi cation for further studies.