Characterisation of extraembryonic endoderm-like cells from mouse embryonic fibroblasts induced using chemicals alone

摘要

Generation of ciXEN cells. Schematic diagram for obtaining ciXEN cells from MEFs using small molecules. Morphological appearances of MEFs, cell colonies (treatment with chemicals for 8 days (8d) and 16 days (16d)) (bar, 100 μm), and epithelial-like cells emerging out of the selected clone (24d) (bar, 200 μm). Numbers of cell clones with different numbers of starting cells: 3w, 4w, and 5w. Effect of Matrigel on the formation of clones from MEFs with 4w initial cells. Co-immunostaining (Foxa2 and Sox17) and phase-contrast image of cell clone (bar, 50 μm). qPCR results for the expression of XEN-related genes and ( ), and fibroblast markers and epithelial markers ( ) from day 0 to day 24. V, VPA; P, Parnate; A, AM580; C, CHIR99021; R, RepSOX; F, forskolin; E, EPZ004777; 3w, 3 × 10 cells; 4w, 4 × 10 cells; 5w, 5 × 10 cells. *  p p t test and two-way ANOVA,  ≥ 3