LiCoO2 particles used in Li-ion batteries induce primary mutagenicity in lung cells via their capacity to generate hydroxyl radicals

摘要

LCO particles induce MN in lung epithelial cells in vitro. Rat lung epithelial cells (RLE, 55556 cells/cm ) were exposed to culture medium (control, CTL), WC-Co, LCO or LTO, and cytotoxicity was assessed after 24 h by the WST-1 assay ( ). The CBPI ( ) was assessed in 500 cells exposed to non-cytotoxic particle concentrations, and the frequency of MN determined in 1000 binucleated cells ( ). Image of a binucleated cell containing a micronucleus designated by the red arrow ( ). *  P P N = 2 for results obtained with 5 μg/ml LCO;  = 4 for all other results with  = 2 for CTL and  = 4 for all other conditions)