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Evaluation of Swabs Transport Media and Specimen Transport Conditions for Optimal Detection of Viruses by PCR

机译:评估用于通过PCR最佳检测病毒的拭子运输介质和标本运输条件

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摘要

Depletion of swabs and viral transport medium during epidemics may prompt the use of unvalidated alternatives. Swabs collected and transported dry or in saline were compared to commercially available swab/medium combinations for PCR detection of influenza, enterovirus, herpes simplex virus, and adenovirus. Each was detected at an ambient temperature (22°C) and 4°C for 7 days. Detection of influenza on dry or saline swabs is important because of its capacity to cause outbreaks involving large numbers of cases.
机译:流行期间棉签和病毒运输介质的耗尽可能促使使用未经验证的替代品。将收集并干燥或在盐水中运输的拭子与市售拭子/培养基组合进行比较,以进行PCR检测流感,肠病毒,单纯疱疹病毒和腺病毒。在环境温度(22°C)和4°C下检测7天。在干燥或盐水拭子上检测流感很重要,因为它具有引起大量病例爆发的能力。

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