lnc9141-a and -b Play a Different Role in Bovine Myoblast Proliferation Apoptosis and Differentiation

摘要

Previously, our transcriptome sequencing revealed that lnc9141 was differentially expressed in muscles of fetal bovine, calf, and adult bovine, which is considered to provide the basis for raising the muscle mass. In this study, we identified lnc9141 characters. has different transcription start sites and 3′ alternative splicing sites of exon 1, producing lnc9141-a and lnc9141-b transcripts that were highly expressed in the heart and lung. Moreover, neither lnc9141-a nor lnc9141-b had the ability to encode proteins. The functions of lnc9141-a and lnc9141-b were explored by cell cycle, 5-ethynyl-2'-deoxyuridine (EdU), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that lnc9141-a or lnc9141-b overexpression decreased the number of myoblasts in the S phase and increased the proportion of cells in the G /G phase. Furthermore, overexpressing lnc9141-a and lnc9141-b respectively downregulated the expression of . However, lnc9141-a or lnc9141-b interference was found to increase the number of S-phase myoblasts, and upregulate and expression. Through Annexin V-FITC/propidium iodide (PI) double staining and the expression of apoptosis marker genes ( , , and ), it was found that lnc9141-b could regulate the expression of gene. Meantime, high expression of lnc9141-b could decrease expression. In addition, the intergenic region between and was 2.3 kb, with a head-to-head orientation. The study also revealed the core regions of the and promoter. Our study demonstrated that both lnc9141-a and -b expression inhibited bovine myoblast proliferation. However, lnc9141-b regulated and expression. The regulatory mechanism of lnc9141-a and lnc9141-b needs to be further explored.