ampPD: An Automated Primer Design Tool for Highly Multiplexed Single-Tube Tiled Amplicon PCR for Resequencing of Tumor Samples

摘要

PCR-based target enrichment is widely used to prepare libraries for next generation sequencing (NGS). Amplicon tiling is often used for assay design but can result in amplicon overlap that reduces assay sensitivity. Pillar Biosciences™ SLIMamp® enrichment technology enables efficient amplicon tiling even with overlapping amplicons. Here, we present an automated primer design tool (ampPD) for amplicon tiling and evaluate the performance of an assay designed against the TP53 tumor suppressor gene.