Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular changes and time

摘要

Schematics of the engineered gene circuit implementing a half adder in bacteria Symbolic and logic representation of the circuit. IPTG and aTc are the two molecules that work as input signals in the system (note that the PLlac or PLtet promoter implements an IMPLY gate), while the expressions of an RFP (XOR gate; from plasmid pRHA40) and a GFP (AND gate; from plasmid pRHA12) constitute the output responses