Infusion of sodium butyrate promotes rumen papillae growth and enhances expression of genes related to rumen epithelial VFA uptake and metabolism in neonatal twin lambs

摘要

The objective of this study was to evaluate the effect of sodium butyrate ( ) infusion on rumen papillae growth and volatile fatty acid ( ) uptake and metabolism in neonatal lambs. Seven pairs of newborn twin lambs were used. Within each pair, lambs were assigned to receive an oral infusion of SB at 0.36 g/kg body weight ( ) (SB, = 7) or the same volume of saline (Con, = 7). Treatments were administered from 10 to 49 d of age, when all lambs were slaughtered. Results showed that the average daily feed intake ( ) of starter, average daily gain ( ), BW of lambs at ages of 5 and 6 wk in SB group were greater ( < 0.05) than those in Con group. Infusion of SB increased ( < 0.05) the concentrations of acetate, butyrate, and total VFA in the rumen fluid and elevated ( < 0.05) the levels of β-hydroxybutyrate acid ( ), insulin-like growth factor-1 ( ), and insulin in plasma. Infusion of SB promoted rumen papillae growth, depicted by higher emptied rumen weight, larger rumen papillae length, width, and surface area, and greater thickness of stratum corneum and total epithelium. Sodium butyrate infusion upregulated ( < 0.05) mRNA expression of , , and ( ), and downregulated ( < 0.05) mRNA expression of and ( ) in the rumen epithelia. Moreover, SB infusion also upregulated ( < 0.05) mRNA expression of ( ), and ( ), and downregulated ( < 0.05) mRNA expression of ( ) in the rumen epithelia. Sodium butyrate infusion also enhanced ( < 0.05) gene expressions of ( ), ( ), ( ), and ( ), while depressed ( < 0.05) mRNA expression of ( ) in the rumen epithelia. Our results suggest that the SB infusion can improve animal performance, promote the ruminal papillae growth, and enhance expression of genes related to ruminal epithelial VFA uptake and metabolism in preweaning twin lambs. These findings provide a better understanding of the molecular mechanism of SB promoting rumen epithelial development and function in preweaning lambs.