Reduced TCA cycle rates at high hydrostatic pressure hinder hydrocarbon degradation and obligate oil degraders in natural deep-sea microbial communities

摘要

Experimental set up. Microbial communities from hydrocarbon-free, marine sediments collected at 1000 m below sea surface level (≈10 MPa) were enriched in a HP range 0.1–20 MPa, using either C or C as sole carbon source. Biomasses from enriched consortia were characterized, including that from a third high HP reactor at 30 MPa inoculated from 20 MPa reactors. Isolation from 10 and 20 MPa reactors supplied with C was conducted, yielding multispecies colonies. These were pooled and tested further in a high HP-adapted synthetic community (HHP-SC) at 0.1 and 10 MPa, using either C or acetate as sole carbon source