Phylogenetic genomic and biogeographic characterization of a novel and ubiquitous marine invertebrate-associated Rickettsiales parasite Candidatus Aquarickettsia rohweri gen. nov. sp. nov

摘要

FISH on sections with general and specific probes. Suspected RLOs, evident in Giemsa-stained section of actinopharynx body wall, including mucocyte among -infected cells in the gastrodermis. , Ten micrometers thick sections, mixed EUB338-I+EUB338-II+EUB338-III (Alexa546, red) and RICK (Alexa488, green) probes co-localized to clusters of bacterial cells of similar size, morphology, and location as in the Giemsa-stained sections. RICK probe localizes to cells in an aggregate (red arrow), and there is no signal from single-base mismatch probe RICKNEG (green). RLO Rickettsiales-like organism clusters, S . Bar = 50 µm; bar = 50 µm; bar = 10 µm; bar = 10 µm