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Impact of a Short Period of Pre-Enrichment on Detection and Bacterial Loads of Methicillin-Resistant Staphylococcus aureus from Screening Specimens

机译:短期预富集对筛选样品中耐甲氧西林金黄色葡萄球菌的检测和细菌负荷的影响

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摘要

We compared the impacts of direct plating on a chromogenic medium and of plating after enrichment (4 h and overnight) on the detection of methicillin-resistant Staphylococcus aureus (MRSA) from 52 patient screening samples. MRSA colony counts for ≈70% of samples after overnight pre-enrichment were >20-fold higher than the counts after the other two treatments. The qualitative differences (sample was MRSA positiveegative) between the results of the direct plating and 4-h pre-enrichment treatments were not significant, indicating no advantage of the latter; however, the number of samples positive for MRSA increased significantly after an overnight sample pre-enrichment (P < 0.005).
机译:我们比较了直接电镀在发色培养基上和富集(4小时和过夜)后电镀对从52例患者筛查样品中耐甲氧西林金黄色葡萄球菌(MRSA)检测的影响。过夜预富集后约70%的样品的MRSA菌落计数比其他两种处理后的计数高20倍以上。直接电镀的结果与4小时预富集处理之间的质量差异(样品为MRSA阳性/阴性)不显着,表明后者没有优势。然而,经过一夜的样品预富集后,MRSA阳性的样品数量显着增加(P <0.005)。

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