Oxathiapiprolin a Novel Chemical Inducer Activates the Plant Disease Resistance

摘要

Oxathiapiprolin was developed as a specific plant pathogenic oomycete inhibitor, previously shown to have highly curative and protective activities against the pepper Phytophthora blight disease under field and greenhouse tests. Therefore, it was hypothesized that oxathiapiprolin might potentially activate the plant disease resistance against pathogen infections. This study investigated the potential and related mechanism of oxathiapiprolin to activate the plant disease resistance using the bacterium pv ( ) and plant interaction as the targeted system. Our results showed that oxathiapiprolin could activate the plant disease resistance against DC3000, a non-target pathogen of oxathiapiprolin, in , tobacco, and tomato plants. Our results also showed the enhanced callose deposition and H O accumulation in the oxathiapiprolin-treated under the induction of flg22 as the pathogen-associated molecular pattern (PAMP) treatment. Furthermore, increased levels of free salicylic acid (SA) and jasmonic acid (JA) were detected in the oxathiapiprolin-treated plants compared to the mock-treated ones under the challenge of DC3000. Besides, the gene expression results confirmed that at 24 h after the infiltration with DC3000, the oxathiapiprolin-treated plants had upregulated expression levels of the respiratory burst oxidase homolog D ( ), JA-responsive gene ( ), and SA-responsive genes ( , , and ) compared to the control. Taken together, oxathiapiprolin is identified as a novel chemical inducer which activates the plant disease resistance against DC3000 by enhancing the callose deposition, H O accumulation, and hormone SA and JA production.