Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot

摘要

Wheat ( L.) is an important staple crop. Sharp eyespot and common root rot are destructive diseases of wheat. Antimicrobial peptides (AMPs) are small peptides with broad-spectrum antimicrobial activity. In this study, we synthesized the gene, encoding DmAMP1, and investigated the antifungal role of DmAMP1W in vitro and in transgenic wheat. Protein electrophoresis analysis and in vitro inhibition results demonstrated that the synthesized correctly translated to the expected peptide DmAMP1W, and the purified peptide inhibited growths of the fungi and , the pathogenic causes of wheat sharp eyespot and common root rot. was introduced into a wheat variety Zhoumai18 via -mediated transformation. The molecular characteristics indicated that could be heritable and expressed in five transgenic wheat lines in T –T generations. Average sharp eyespot infection types of these five transgenic wheat lines in T –T generations decreased 0.69–1.54 and 0.40–0.82 compared with non-transformed Zhoumai18, respectively. Average common root rot infection types of these transgenic lines and non-transformed Zhoumai18 were 1.23–1.48 and 2.27, respectively. These results indicated that DmAMP1W-expressing transgenic wheat lines displayed enhanced-resistance to both sharp eyespot and common root rot. This study provides new broad-spectrum antifungal resources for wheat breeding.