Representative picture of agarose gel electrophoresis of serum proteins followed by staining with BNA for detection of esterase activity. The gel is incubated with (a) 75 µM BNA (in ethanol), (b) 75 µM BNA (in acetone), (c) 1 mM BNA (in ethanol), (d) 1 mM BNA (in acetone). The densitometry analysis of the background of the gel (→). Mean ± SD (n = 6) of the background density is shown in (e) for (a) & (b) and (f) for (c) & (d) respectively (Y-axis in square pixel where 96 pixel = 1 inch). *** value less than 0.0001. Representative picture of native PAGE of plasma proteins followed by staining with BNA for detection of esterase activity. The gel is incubated with (a) 75 µM BNA (in ethanol), (b) 75 µM BNA (in acetone), (c) 1 mM BNA (in ethanol), (d) 1 mM BNA (in acetone). The densitometry analysis of the background of the gel (→). Mean ± SD (n = 6) of the background density is shown in (e) for (a) & (b) and (f) for (c) & (d) respectively (Y-axis in square pixel where 96 pixel = 1 inch). *** value less than 0.0001
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机译:血清蛋白的琼脂糖凝胶电泳,然后用BNA染色以检测酯酶活性的代表性图片。将该凝胶与(a)75 µM BNA(在乙醇中),(b)75 µM BNA(在丙酮中),(c)1 mM BNA(在乙醇中),(d)1 mM BNA(在丙酮中)一起孵育。凝胶背景的密度分析(→)。 (a)和(b)的(e)和(c)和(d)的(f)分别在(e)中显示背景密度的平均值±SD(n == 6)(Y轴以正方形像素表示,其中96 pixel = 1英尺)。 ***值小于0.0001。血浆蛋白天然PAGE的代表性图片,然后用BNA染色以检测酯酶活性。将该凝胶与(a)75 µM BNA(在乙醇中),(b)75 µM BNA(在丙酮中),(c)1 mM BNA(在乙醇中),(d)1 mM BNA(在丙酮中)一起孵育。凝胶背景的密度分析(→)。 (a)和(b)的(e)和(c)和(d)的(f)分别在(e)中显示背景密度的平均值±SD(n == 6)(Y轴以正方形像素表示,其中96 pixel = 1英尺)。 ***值小于0.0001
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