Interleukin‐6 signaling requires only few IL‐6 molecules: Relation to physiological concentrations of extracellular IL‐6

摘要

The aim of this study was to give quantitative insight into the number of cytokine molecules needed to activate a target cell and relate this to the physiological consequences of the amounts of cytokines typically detectable in humans. As a model system blood interleukin‐6 (IL‐6) was chosen since this cytokine is one of the most studied and clinically monitored cytokines, and because of the tools for the present investigations such as fully bioactive iodinated recombinant IL‐6, cellular cytokine binding assays, and bioassays have been thoroughly validated.