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CsWAKL08 a pathogen-induced wall-associated receptor-like kinase in sweet orange confers resistance to citrus bacterial canker via ROS control and JA signaling

机译:CsWAKL08一种病原体诱导的甜橙中的壁相关受体样激酶通过ROS控制和JA信号转导赋予柑橘类细菌性溃疡病抗性

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摘要

Genome-wide annotation and Xcc-induced expression profiling of the CsWAKL family. Phylogeny of CsWAKLs. The full-length protein sequences were used for the neighbor-joining tree with MEGA V7.2 based on a bootstrap model (bootstrap = 100). The functional domains of 21 CsWAKLs as predicted using Pfam V32. Different domains are represented by differently colored rounded rectangles. The conserved motifs of CsWAKLs as determined using MEME V5.1. Different expression profiles of CsWAKLs induced by Xcc in Wanjincheng ( ) and Calamondin ( ) were detected via qRT-PCR with CsActin (GenBank: ) as a normalization control. Samples were collected at 0–48 hpi (hours post inoculation). In the heatmap, green and red correspond to low and high expression levels, respectively
机译:CsWAKL家族的全基因组注释和Xcc诱导的表达谱。 CsWAKLs的系统发育。全长蛋白质序列被用于基于自举模型(bootstrap = 100)的带有MEGA V7.2的邻居连接树。使用Pfam V32预测的21个CsWAKL的功能域。不同的域用不同颜色的圆角矩形表示。使用MEME V5.1确定的CsWAKL保守基序。通过以CsActin(GenBank :)为标准化对照的qRT-PCR检测了Xcc在万金城()和Calamondin()中诱导的CsWAKLs的不同表达谱。在0-48 hpi(接种后数小时)收集样品。在热图中,绿色和红色分别对应于低表达水平和高表达水平

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