Detailed data from experimentally-induced mastitis in ewes with the aim to evaluate cathelicidin-1 in milk

摘要

Bacteriological, cytological and proteomics data have been obtained from ewes in two experiments, after intramammary challenge with or . Animals were sampled before and sequentially after challenge. Conventional techniques were employed for bacterial isolation and somatic cell counting in milk samples; milk whey samples were subjected to proteomics evaluation by using two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. There was a correlation between leucocyte content and cathelicidin-1 spot densities in milk samples, although the protein was detected in milk earlier than the increase in leucocyte content. There was also a significant association between presence of mastitis in a mammary gland and detection of cathelicidin-1 in the respective milk sample; the degree of association was greater during the first 24 h post-inoculation. The data are further discussed in the research article “Detection of cathelicidin-1 in the milk as an early indicator of mastitis in ewes” [1].