C57/B6 mice were subjected to hepatic IRI model. Gsdmd-full, Gsdmd-N, caspase-1, and cleaved caspase-1 expression in liver tissues were detected by Western blotting. Serum levels of IL-1β, caspase-1 activity, and mRNA levels of IL-1β were measured in liver tissues. Representative images of caspase-1 staining (scale bar, 30 μm), the area of caspase-1-positive cells in the livers were also determined. All data are shown as the mean ± SD, (sham) = 4 mice per group, (IRI) = 6 mice per group. *** P P
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机译:对C57 / B6小鼠进行肝IRI模型。通过Western印迹检测肝组织中Gsdmd-full,Gsdmd-N,caspase-1和裂解的caspase-1表达。在肝组织中测量血清IL-1β水平,caspase-1活性和IL-1βmRNA水平。还测定了caspase-1染色的代表性图像(比例尺,30μm),肝脏中caspase-1阳性细胞的面积。所有数据显示为平均值±SD,(假)=每组4只小鼠,(IRI)=每组6只小鼠。 *** P P
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