Kinesin light chain 4 as a new target for lung cancer chemoresistance via targeted inhibition of checkpoint kinases in the DNA repair network

摘要

Cell viability was measured every 12 h after treatment with 10 μM cisplatin. Death of cells treated with or without 10 μM cisplatin during 12 h intervals. After cisplatin treatment for 24 h, the cells were fixed with 4% paraformaldehyde and immunostained using an antibody targeting γH2AX (DNA damage marker); DNA was visualized using DAPI staining. Protein levels of KLC4, cleaved PARP, and active caspase-3 were determined using western blotting after cisplatin treatment. – Cells treated with etoposide, gefitinib, or Taxol, respectively. Twenty-four hours after the drug treatments, the cells were analyzed using cell viability assay, followed by ELISA.