Karyopherin α2-dependent import of E2F1 and TFDP1 maintains protumorigenic stathmin expression in liver cancer

摘要

Stathmin is downregulated upon KPNA2 depletion. Workflow of LC-MS/MS analysis. HLE cells were harvested 72 h after control (Ctrl.) or KPNA2 siRNA treatment (  = 3). Volcano-Plot illustrates the resulting log fold-changes (KPNA2/Ctrl. siRNA) and corresponding log -values of 1759 proteins being quantified by the LC-MS/MS analysis. Horizontal dotted line  = 0.01; vertical dotted lines log fold-change 0.8 or − 0.8; green dot: KPNA2; red dot: stathmin. HLE and HLF cells were siRNA-treated and harvested as described in ( ). Lysates were immunoblotted using the indicated antibodies. HLE and HLF cells were treated as described in ( ) and expression was analyzed by qRT-PCR. (HLE:  = 5,  n = 4,