Regional myocardial strain analysis via 2D speckle tracking echocardiography: validation with sonomicrometry and correlation with regional blood flow in the presence of graded coronary stenoses and dobutamine stress

摘要

Experimental open chest preparation with dissection of the LAD distal to the first diagonal and incorporation of a Doppler flow probe, hydraulic occluder, and snare. The 16-crystal sonomicrometer array contains pairs of subepicardial and subendocardial crystals which define cubic regions in the ischemic, border, and remote LAD territories. A left atrial catheter was also inserted for microsphere administration. Schematic of experimental protocol. Data acquisitions at each experimental condition include invasive blood pressures, doppler LAD flow measurements, 2D echocardiography, sonimicrometry, and microsphere blood flow analysis