miR-363 suppresses the proliferation migration and invasion of clear cell renal cell carcinoma by downregulating S1PR1

摘要

Expression and prognostic significance of miR-363 in ccRCC and its relationship with S1PR1 expression. miR-363 expression levels were significantly downregulated in ccRCC tissues compared to adjacent normal tissues. Comparison of miR-363 expression levels between subgroups of patients by clinical stage, T stage and Fuhrman grade. miR-363 expression in normal renal cell lines and various RCC cell lines. Kaplan–Meier analysis of ccRCC patients in the low miR-363 group (n = 39) and the high miR-363 group (n = 38) with regard to disease-free survival. S1PR1 expression levels were markedly upregulated in ccRCC tissues compared to adjacent normal tissues. and S1PR1 protein expression in normal renal cell lines and various RCC cell lines. Negative correlation between S1PR1 mRNA levels and miR-363 levels in ccRCC tissues (n = 77, r = −0.509, P k Quantitative analysis of S1PR1 relative protein expression in ccRCC tissues and adjacent normal tissues by western blot. Histoscores of S1PR1 in ccRCC tissues and adjacent normal tissues based on IHC. Western blot images showed that S1PR1 is upregulated at the protein level in ccRCC tissues, consistent with alterations in mRNA levels in clinical samples. Representative IHC staining images of S1PR1 in ccRCC tissues and their paired normal tissues. Data are presented as the mean ± SD. (*P