Biomarkers to quantify cell migration characteristics

摘要

Cell movement in 2D and 3D conditions, and cellular elasticity. Schematic diagram of a single cell tracking system using an optical microscope. Two-dimensional cell movement was tracked in Cartesian coordinates at 1 min intervals for 8 h. At each time point, the cell location was determined based on the nucleus. Cell displacement was converted to a scalar quantity. Comparison between cells of traveling distance on a 2D substrate. Three-dimensional migration ability was evaluated with a transwell membrane assay. The cells migrated through an 8 µm pore were stained in blue. Relative number of migrated cells. Representative FD curve of the deformable cells. The approach and retraction curve are marked with black and blue rectangular points, respectively. Approach curve was fitted to the Sneddon model to obtain the Young’s modulus of the cell. Determined Young’s modulus of the cells. Each value represents mean ± SEM (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001)