Focal adhesion protein Kindlin-2 regulates bone homeostasis in mice

摘要

Immunofluorescence staining of tibial sections of 4-month-old male mice and control littermates with an anti-Kindlin-2 antibody. Scale bar, 80 μm. Animal growth curves.  = 2–6 male mice per group. *  t test. The results are expressed as the mean ± standard deviation (s.d.). Hematoxylin and eosin staining of tibial ( ) or lumbar spine (L4) ( ) sections from 2-month-old male mice and their control littermates. Scale bar: 160 μm ( ) or 400 μm ( ). Three-dimensional (3D) reconstruction from microcomputed tomography (μCT) scans of the distal femurs of mice and their control littermates with the indicated ages and sexes. – Quantitative analyses of bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), cortical thickness (Cort.Th), and bone mineral density (BMD) of distal femurs of mice and their control littermates with the indicated ages and sexes.  = 4, 14-month-old male mice;  = 6, 14-month-old male control mice;  = 9, 6-month-old male control mice;  = 7 mice in the remaining groups. *  P P t test. The results are expressed as the mean ± s.d.