The DNA methyltransferase inhibitor guadecitabine targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer

摘要

Guadecitabine treatment results in smaller tumors and a reduction in myeloid cells. 4 T1 tumor-bearing mice were untreated or treated with guadecitabine on days 10–13. Final volume of excised tumors on day 16. Representative images of frozen tumor sections stained with TUNEL to detect apoptotic cells. Total cellularity (top) and number of MDSCs (bottom) from spleen, bone marrow, and blood. Surface expression of APC costimulatory markers on splenic MDSCs. Significance determined using student’s unpaired T test (a), ANOVA with Tukey’s (c-e) or Sidak’s (f) multiple comparison tests. Error bars represent SD. ns = not significant; **: valuep valuep value