The small Ca2+-binding protein CSE links Ca2+ signalling with nitrogen metabolism and filament integrity in Anabaena sp. PCC 7120

摘要

Construction and verification of the mutant. Scheme of the vector construct, with a kanamycineomycin resistance cassette (KmR/NmR) replacing the gene. Polymerase chain reaction (PCR) confirmation of two independently-obtained, fully segregated clones. Amplification of the gene with its upstream and downstream flanking regions in wild-type (WT) resulted in a PCR product of 3593 bp. In the clones, replacement of with KmR/NmR resulted in a larger PCR product of 4075 bp. No traces of the WT PCR product in the clones indicated full segregation of the mutant. Expression of in WT and clone 1 normalised to the expression of the reference gene . Error bars indicate the standard deviation from three biological replicates (  = 3)