Optimised production of an anti-fungal antibody in Solanaceae hairy roots to develop new formulations against Candida albicans

摘要

Generation of and HR expressing scFvFc 2G8. Schematic representation of construct containing the scFv format of the mAb 2G8 fused to the Fc-encoding sequence of the human IgG1 chain or vector used for irrelevant control (pBIc-). SP: signal peptide sequence derived from an embryonic immunoglobulin to direct the recombinant antibody to the secretory pathway [ ]; Ω: TMV translational enhancer [ ]; 35S prom: Cauliflower Mosaic Virus 35S promoter; Nos ter: Nopaline synthetase terminator sequence. Western blot analysis of TSP (5 μg) extracted from HR expressing scFvFc 2G8 or C- separated on a 10% Tris/glycine gel by SDS-PAGE in reducing ( ) or non-reducing ( ) conditions. C+: 25 ng of purified scFvFc 2G8. Blots were detected with anti-human IgG1-antibody conjugated to HRP