Expert curation of the human and mouse olfactory receptor gene repertoires identifies conserved coding regions split across two exons

摘要

Olfactory Receptor annotation pipeline. Flow diagram showing the steps taken to annotate all OR loci of the human and mouse genomes. Specific databases and programs used are indicated in grey. The pipeline consists of two major tasks: 1) curating all available annotation for OR genes, as depicted on the purple-shaded steps; and 2) integrating transcriptional evidence from RNAseq data and mRNA, EST and PacBio clones, to construct gene models including untranslated regions, as shown on the blue-shaded steps. Results from both tasks were integrated into a comprehensive annotation of the human and mouse OR repertoires. These were subsequently added to the GENCODE project. 7TM = seven transmembrane domain