Tracy: basecalling alignment assembly and deconvolution of sanger chromatogram trace files

摘要

Sanger sequencing has a long history in molecular biology and it remains indispensable for many routine tasks like the sequencing of single genes, cloned plasmids, expression constructs or PCR products [ ]. Many tools facilitate the comparison of traces with a short reference subsequence, but they lack support to align traces across entire genomes, to deconvolute mutations or to patch a reference sequence based on trace information. Automatisation of these standard tasks avoids misinterpretation of mutations and aids the researchers to focus on the critical mutations instead of inspecting hundreds of chromatogram peaks by eye. Beyond these routine chromatogram evaluation tasks that require a graphical trace analysis application, large-scale genome editing and clinical resequencing projects demand a flexible and scalable command-line application that can be integrated into automated workflows.