Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem

摘要

Reaction scheme of cellulose phosphorolysis. Cellulosic biomass was enzymatic phosphorylated via in vitro synthetic enzymatic biosystems to manufacturing biochemicals or biofuels. In the free enzyme mixture, CDP prefers to convert cellodextrin with a degree of polymerization of  − 1 (G ) and G1P to G and inorganic phosphate (P ) despite the presence of PGM. The enzyme complex containing CDP and PGM was supposed to accelerate the degradation of cellodextrin to G6P. cellodextrin phosphorylase, phosphoglucomutase, cellodextrin, glucose 1-phosphate, glucose 6-phosphate, inorganic phosphate