Distribution and diversity of olefins and olefin-biosynthesis genes in Gram-positive bacteria

摘要

Olefin biosynthesis via the ole pathway according to Christenson et al. [ ]. The thiolase OleA converts two acyl-CoAs in a non-decarboxylating “head-to-head” Claisen condensation to a β-keto acid. In a NADPH-dependent reaction, the dehydrogenase OleD reduces the β-keto acid to the corresponding β-hydroxy acid. The β-lactone synthetase OleC converts the β-hydroxy acid into a heat-unstable β-lactone. Finally, OleB decarboxylates the β-lactone to the cis olefin