CID1067700 a late endosome GTPase Rab7 receptor antagonist attenuates brain atrophy improves neurologic deficits and inhibits reactive astrogliosis in rat ischemic stroke

摘要

The time-course changes of GFAP, Phosphacan and Rab7 protein. Representative images of GFAP, Phosphacan and Rab7 in the peri-infarct zones of the sham or cerebral ischemic cortex at 2, 4, 6 h, 1, 3, 7, and 14 days after reperfusion following tMCAO for 90 min with Western blotting analysis. Columns represent quantitative analysis of immunoblots respectively. β-actin protein was used as a loading control. Mean ± SD.    3.  P b Representative images of Rab7, GFAP, and Hoechst staining in the peri-infarct zones of the sham or cerebral ischemic cortex at 7 days after reperfusion following tMCAO for 90 min (Rab7: green; GFAP: red; Hoechst: blue). Quantification of green fluorescence intensity of Rab7 immunostaining. Quantification of red fluorescence intensity of GFAP immunostaining. Mean ± SD.  = 6.  c Representative images of GFAP, Phosphacan and Rab7 at OGD 6 h and reoxygenaration for 6, 12, and 24 h with Western blotting analysis. Columns represent quantitative analysis of immunoblots respectively. β-actin protein was used as a loading control. Mean ± SD.    3.  ##